Accelerated Macromolecular Solution Shape Recovery from Small Angle Scattering Data

Small-angle scattering studies of macromolecular solutions

In recent years, major progress has been achieved in developing novel approaches to interpret small-angle scattering data from solutions of biological macromolecules in terms of three-dimensional models. These advanced methods include: ab initio low-resolution shape and domain structure determination; modelling of quaternary structure by rigid-body refinement; simultaneous analysis of multiple ...

Small Angle X-ray Scattering from Macromolecular Solutions and Nanoparticles

Small-angle X-ray scattering (SAXS) experiences a renaissance in the studies of macromolecular solutions allowing one to study the structure of native particles and to rapidly analyze structural changes in response to variations in external conditions [1]. Novel data analysis methods significantly enhanced resolution and reliability of structural models provided by the technique. Emerging autom...

ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions

ATSAS is a comprehensive software suite for the analysis of small-angle scattering data from dilute solutions of biological macromolecules or nanoparticles. It contains applications for primary data processing and assessment, ab initio bead modelling, and model validation, as well as methods for the analysis of flexibility and mixtures. In addition, approaches are supported that utilize informa...

Investigating Macromolecular Complexes in Solution by Small Angle X-Ray Scattering

Reconstructing three-dimensional shape envelopes from time-resolved small-angle X-ray scattering data.

Modern computing power has made it possible to reconstruct low-resolution, three-dimensional shapes from solution small-angle X-ray scattering (SAXS) data on biomolecules without a priori knowledge of the structure. In conjunction with rapid mixing techniques, SAXS has been applied to time resolve conformational changes accompanying important biological processes, such as biomolecular folding. ...